Generator

Part:BBa_K1100010:Design

Designed by: YingQi Lou   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 12:21, 21 September 2013 by Shuqidu (Talk | contribs) (→‎Source)


Csy4 generator (with terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164


Design Notes

To generate Csy4 protein


Source

The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, BOO15 from Kit.

References