Regulatory
FUS1 pr

Part:BBa_K1154001:Design

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-17)
Revision as of 02:57, 21 September 2013 by RicRHIT (Talk | contribs) (→‎Source)

Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence is from Saccharomyces cerevisiae Reference Strain S288C chromosome III coordinates 71603 to 71803, in the promoter region of the FUS1/YCL027W coding sequence. It is very similar to the FUS1 promoter well characterized by the 2012 TU-Delft iGEM Team (see Part:BBa_K775004). However, the two sequences do differ:

Align BBa K775004 BBa K1154001.JPG

Source

Sequence is from the genome of Saccharomyces cerevisiae Reference Strain S288C.

References

McCaffrey G, Clay FJ, Kelsay K, and Sprague GF Jr (1987) Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7 (8), 2680-2690.