Coding

Part:BBa_K1166005

Designed by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero   Group: iGEM13_TecMonterrey   (2013-09-17)
Revision as of 16:50, 20 September 2013 by LuisFernandoCG (Talk | contribs)

TAT-Apoptin

Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin is a protein from the Chicken Anemia Virus known to cause apoptosis in more than 70 human cancer cell lines only using a pathway independent from P53 by a phosphorylation in the Threonine 108.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None