Coding

Part:BBa_K1017301

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 15:39, 20 September 2013 by Amay (Talk | contribs)

Cph8(cph1-envZ)

NCTU Formosa part 3.png

Cph8 is a chimeric light receptor. It is a fusion of the photoreceptor Cph1 and the EnvZ histidine kinase. The light sensing unit is connected to transcription via the EnvZ-OmpR signaling pathway, phosphorylated OmpR acting as a transcription factor of PompC. Far red light activates the EnvZ kinase which results in the phosphorylation of OmpR and its activation as a transcription factor. On the other hand, red light deactivates the kinase and therefore the genes downstream can not be expressed.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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