Composite

Part:BBa_K1154000:Design

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-16)
Revision as of 11:46, 20 September 2013 by RicRHIT (Talk | contribs) (→‎Source)

Mating pheromone-induced IGPD and constitutive LDH expression in Yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This commercially synthesized construct was designed for high codon bias in Saccharomyces cerevisiae and conformity with current Registry standards. Its component sequences can be found in the Registry (see Source). However, assembling those sequences using the Composite Parts Tool will not produce a construct with sequence identical to that submitted here.

Source

This sequence was commercially synthesized. Component sequences can be found in the Registry: Yeast FUS1 promoter (BBa_K1154001) Yeast TEF2 promoter (BBa_K165037) Yeast ADH1 terminator (BBa_J63002) Yeast imidazoleglycerol-phosphate dehydratase (IGPD)coding sequence (BBa_K1154002) Human lactate dehydrogenase A (LDHA) coding sequence (BBa_K1154003) However, assembling these sequences using the Composite Parts Tool will not produce a sequence identical to the one submitted here.

References