Composite

Part:BBa_K1173501

Designed by: paul james   Group: iGEM13_Exeter   (2013-09-20)
Revision as of 10:12, 20 September 2013 by Registry (Talk | contribs)

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CcaS, CcaR, PcpcG2, cI(lambda), magenta

This is a composite part which is responsive to green light and controls the production of a magenta pigment. CcaS is a trans-membrane protein which responds to green light by phosphorylating CcaR when green light is present. CcaR-P then binds to a specific promoter (BBa_K592003), which allows the transcription of the cI protein. This in turn binds to a specific promoter, which prevents the transcription of a gene coding for a magenta pigment (BBa_K592012). Overall, this means that exposure of the cells to green light will halt the synthesis of magenta pigment. Inversely, lack of exposure to green light will allow the synthesis of magenta pigment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 667
    Illegal NheI site found at 2478
    Illegal NheI site found at 2501
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2934
    Illegal XhoI site found at 2313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1331
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None