Coding
tetA(C)b
Part:BBa_J31006
Designed by: Sabriya Rosemond, Erin Zwack Group: iGEM06_Davidson (2006-07-12)
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/selectionmarker/antibioticresistance
function | Rep |
ligands | tetracycline |
protein | TetA(C)-bkw |
swisspro | P02981 |