Composite

Part:BBa_K1132031

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-19)
Revision as of 08:02, 20 September 2013 by Mesnageclem (Talk | contribs)

Test of the AND gate (BBa_K1132001) with RFP

This BioBrick is design to test the AND gate (BBa_K1132001) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been assembled with our gate.

2031.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 286
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 295
    Illegal AgeI site found at 1091
    Illegal AgeI site found at 1203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 315
    Illegal BsaI.rc site found at 476


[edit]
Categories
Parameters
None