Part:BBa_K1062000

Guide RNA (gRNA) target for RFP

This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as:

1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project

2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.

Conjugation Project

Synthetic Circuit