Generator

Part:BBa_K1049002:Experinece

Designed by: Yohei TAMIAKI   Group: iGEM13_KIT-Kyoto   (2013-09-19)
Revision as of 02:14, 20 September 2013 by Okaeri007 (Talk | contribs)

Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis. ATF2.png