Generator
Part:BBa_K1049002:Experience
Designed by: Yohei TAMIAKI Group: iGEM13_KIT-Kyoto (2013-09-19)
Revision as of 02:08, 20 September 2013 by Okaeri007 (Talk | contribs) (→Applications of BBa_K1049002)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1049002
Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis.
User Reviews
UNIQ5a47d555d8da49df-partinfo-00000000-QINU UNIQ5a47d555d8da49df-partinfo-00000001-QINU