Coding

Part:BBa_K1076003:Design

Designed by: Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)
Revision as of 00:15, 20 September 2013 by Lais (Talk | contribs) (→‎Design Notes)


FadD, Long-chain-fatty-acid-CoA ligase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 163


Design Notes

We chosed this ORF gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5-152001. Should also be assembled with promoter, RBS and terminator.

Source

From Shewanella putrefaciens genomic sequence

References