Coding

Part:BBa_K1076003

Designed by: Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)
Revision as of 23:58, 19 September 2013 by Lais (Talk | contribs)

FadD, Long-chain-fatty-acid-CoA ligase

FadD ligates CoA in fatty acid chain preparing it to get into the Beta-Oxidation metabolic pathway.We are expressing this coding sequence (ORF) in order to enhance the long chain fatty acids intake by Shewanella putrefaciens to increase B-oxidation and therefore ATP and H+ production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 163


[edit]
Categories
Parameters
None