Device

Part:BBa_K1088027

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-09-19)
Revision as of 19:45, 19 September 2013 by Registry (Talk | contribs)

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Dxs from B. subtilis with lac promoter and lacI (IPTG inducible)

The part consist of the dxs gene derived from B. subtilis under the control of the lac promoter and has a strong RBS.

To repress expression from the lac promoter, the lacI gene under a constitutive promoter, with a strong RBS and a efficient terminator is placed counter-clockwise to the reporter fusion. The repression can then be relieved with addition of IPTG, which binds and inhibits the function of LacI.

The purpose of the brick is to increase the flow through the MEP pathway upon addition of IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
    Illegal NgoMIV site found at 2417
    Illegal AgeI site found at 2310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2259
    Illegal SapI.rc site found at 2958


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