DNA
RE

Part:BBa_J3101:Design

Designed by: Erin Zwack, Sabriya Rosemond   Group: iGEM06_Davidson   (2006-06-02)
Revision as of 15:52, 12 July 2006 by Erzwack (Talk | contribs) (→‎Design Notes)


Recombinational Enhancer (RE) for Hin/Hix inverting


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The base pair change of a T from a C occurred during cloning but is located in the spacer region between the fis binding sites; therefore, we believe that it will still work.

The insertion is right before the biobrick ends and several bases after the distal fis binding site; therefore, we believe it will function correctly.

We chose this RE to use because after cloning it retained functional full biobricks and the mutations do not seem harmful to function. The other RE that were cloned retained the proper RE sequence but lost critical cut sites in the biobricks.


RE is cloned in plasmid pSB1A2.

Source

References