Reporter

Part:BBa_K1041001:Design

Designed by: NRP UEA   Group: iGEM13_NRP-UEA-Norwich   (2013-08-08)
Revision as of 19:19, 19 September 2013 by Holusac (Talk | contribs) (→‎Design Notes)


Neomycin Resistance Coding Device + AntG promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 816


Design Notes

Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This facilitated cloning of the biobrick Bba_K1041002

Source

BBa_J04450

References