Composite

Part:BBa_K1111007:Design

Designed by: Luisa Spisak, Marcelle Isaline Arrigo   Group: iGEM13_EPF_Lausanne   (2013-09-19)
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MMP2 under the control of the pBAD/araC arabinose inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1645
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The MMP2 gene is expressed when arabinose is added.


Source

The MMP2 sequence is from Homo Sapiens

References