Composite

Part:BBa_K1154000

Designed by: Adam Nighswander, Tanner Reeb and Bianca Maled   Group: iGEM13_RHIT   (2013-09-16)
Revision as of 11:34, 19 September 2013 by RicRHIT (Talk | contribs)

Mating pheromone-induced IGPD and constitutive LDH expression in Yeast

Synthetic DNA optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast HIS3 gene, which encodes imidazoleglycerol-phosphate dehydratase, under control of the mating pheromone-induced FUS1 promoter. It also harbors a gene encoding human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None