Reporter

Part:BBa_K1144007

Designed by: Silvia J Cañas   Group: iGEM13_Colombia_Uniandes   (2013-09-18)
Revision as of 03:38, 19 September 2013 by Registry (Talk | contribs)

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GAL4 (6UAS) activation reporter

The level of expression from the GAL1 promoter (Saccharomyces cerevisiae) is enhanced due to the presence of UAS sites. We fused a modified pGAL1 (6UAS) with a KOZAC sequence, mCherry and the HHO1 yeast terminator to asses the level of expression due to the activacion of GAL4 binding domain

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None