Composite
Part:BBa_K1140003
Designed by: Janssel Reyes del Castillo Group: iGEM13_UANL_Mty-Mexico (2013-09-08)
Revision as of 01:19, 19 September 2013 by GilbertoRdz (Talk | contribs)
pCons + 37 oC RNA thermometer + LacI (LVA)
A construction that has a gene coding for the LacI transcription factor, which also has a LVA degradation tag. The LacI gene is transcribed through a constitutive promoter and a thermolabile RBS with an optimal translation temperature of 37°C. Two termination sites were added at the 3’ end of the gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 44
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
direction | Forward |
function | Repressor |
protein | LacI |
tag | LVA |