Coding

Part:BBa_K1020006:Design

Designed by: Yue Liu   Group: iGEM13_Tianjin   (2013-09-10)
Revision as of 15:50, 18 September 2013 by Ggjyliuyue (Talk | contribs) (→‎Design Notes)


YbhGFSR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1270
    Illegal BglII site found at 3160
    Illegal BglII site found at 4514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2943
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3858


Design Notes

A complete sequence of ybhGFSR transporter and the native E.coli ybhG start codon being changed from GTG to ATG.

Source

E.coli MG1655

References