Composite

Part:BBa_K1020005:Design

Designed by: Yue Liu   Group: iGEM13_Tianjin   (2013-09-10)
Revision as of 15:03, 18 September 2013 by Ggjyliuyue (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Alkane Marker--Device for detection of various alkanes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1028
    Illegal NheI site found at 1051
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 752
    Illegal AgeI site found at 864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1821


Design Notes

We chooce BBa_J23103 as the promoter of AlkR gene, because we assume that overexpress AklR protein will have a negative influence on cell's growth.

Source

Acinetobacter baylyi ADP1

References