Device

Part:BBa_K1065309

Designed by: Fabio Digiacomo   Group: iGEM13_UNITN-Trento   (2013-09-18)
Revision as of 12:10, 18 September 2013 by Registry (Talk | contribs)

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light regulated circuit producing amilGFP+EFE at dark

This part includes blue light sensor YF1, its RR fixJ,PfixK2,the reporter amilGFP and EFE+terminators: everything is under the regulation of Plac. under blue light fixJ is phosphorilated and inhibits the activity of Pfixk2 consisting in production of amilGFP+EFE only at dark.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 950
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1755
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1630
    Illegal AgeI site found at 4060
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1519
    Illegal BsaI.rc site found at 383


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Categories
Parameters
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