Regulatory
AraC

Part:BBa_K1088017:Design

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-09-16)
Revision as of 11:22, 18 September 2013 by Andkj11 (Talk | contribs) (→‎Design Notes)


AraC device (constitutive promoter, RBS and terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The constitutive promoter was used along with a strong RBS to ensure a constant high level of AraC protein. A strong terminator was used to prevent transcription of downstream genes.

Source

>gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655

References