Composite

Part:BBa_K1022116

Designed by: Maithili Krishnan   Group: iGEM13_TU-Delft   (2013-09-18)
Revision as of 08:27, 18 September 2013 by Registry (Talk | contribs)

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pBAD: Ulp: TT: pT7: RBS: His6- SUMO: MaximinH5

This bio brick codes for the promoter pBAD and ulp protease followed by the promoter pT7 and His6-SUMO tag on the Maximin H5 peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
    Illegal NheI site found at 938
    Illegal NotI site found at 714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1062
    Illegal BamHI site found at 683
    Illegal XhoI site found at 692
    Illegal XhoI site found at 723
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1247
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apBAD: Ulp: TT: pT7: RBS: His6- SUMO: MaximinH5