Composite

Part:BBa_K1017726:Design

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)
Revision as of 06:50, 18 September 2013 by Amay (Talk | contribs)

Pcons+B0030+pcya+B0032+ho1+B0030


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 833
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is which we use to change heme into phycocyanobilin(PCB). We use the two different RBS to prevent Homologous recombination, which will cause the mutation of this part.

Source

assemble parts form iGEM distribution I115008, I115009, I115010

References