Coding

Part:BBa_K1166005

Designed by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero   Group: iGEM13_TecMonterrey   (2013-09-17)
Revision as of 19:54, 17 September 2013 by Registry (Talk | contribs)

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TAT-Apoptin

Codes for the T7 expression cassette for Apoptin composed of a N-terminal TAT translocation peptide, followed by a linker (GGGGS), a histidine tag (6x) and by the same linker fused to Apoptin. Apoptin is a protein from the Chicken Anemia Virus known to cause apoptosis in human cancer cell lines only using a pathway independent from P53 by a phosphorilation in the Threonine 108.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None