Reporter
Part:BBa_K1113600
Designed by: Sebastin Alvarez Group: iGEM13_UC_Chile (2013-09-16)
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag.
This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme which deliver into the carboxysome and the ssrA degradation tag which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will “protect” the fluorescens, in any other case this will be degraded by the protease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |