Coding
EbfS Aa

Part:BBa_K1060002:Design

Designed by: Ingmar Claes, Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-17)
Revision as of 13:09, 17 September 2013 by IngmarClaes (Talk | contribs) (→‎References)

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EBF synthase from Artemisia annua


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1306
    Illegal BamHI site found at 615
    Illegal XhoI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

An EcoRI site (513A>C)was removed by site directed mutagenesis


Source

Artemisia annua genomic DNA (Picaud et al. 2005)

References

Francis, F., Martin, T., Lognay, G., and Haubruge, E. (2005). Role of (E)-b-farnesene in systematic aphid prey location by Episyrphusbalteatus larvae (Diptera: Syrphidae). European J. of Entomol., 102:431-436

Kunert, G., Otto, S., Röse, S.R., Gershenzon, J., and Weisser, W.W. (2005). Alarm pheromones mediates production of winged dispersal morphs in aphids. Ecology letters, 8:596-603

Picaud S, Brodelius M, Brodelius PE. (2005). Expression, purification and characterization of recombinant (E)-beta-farnesene synthase from Artemisia annua. Phytochemistry, 6(9):961-7.