Composite

Part:BBa_K1073029

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 12:57, 17 September 2013 by Registry (Talk | contribs)

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Inducible ampicillin resistance cassette + LuxR expression cassette

In complex with the inducer 3-oxo-hexanoyl-HSL, the activator LuxR binds to the Lux promoter and enables expression. This construct provides all the parts required to express the gene behind the Lux promoter. Only the inducer 3-oxo-hexanoyl-HSL is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1098
    Illegal NheI site found at 1121
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None