Reporter

Part:BBa_K1041001:Experience

Designed by: NRP UEA   Group: iGEM13_NRP-UEA-Norwich   (2013-08-08)
Revision as of 11:08, 17 September 2013 by Holusac (Talk | contribs) (→‎Applications of BBa_K1041001)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of BBa_J04450 using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part Bba_K1041002.

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2

User Reviews

UNIQ02c3679c170e1a15-partinfo-00000000-QINU UNIQ02c3679c170e1a15-partinfo-00000001-QINU

User Reviews

UNIQ02c3679c170e1a15-partinfo-00000002-QINU UNIQ02c3679c170e1a15-partinfo-00000003-QINU