Coding

Part:BBa_K1159206

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-17)
Revision as of 06:07, 17 September 2013 by Registry (Talk | contribs)

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Membrane-anchored SpyCatcher in RFC[25] N-Part

This part codes for a membrane-anchored version of SpyCatcher BBa_K1159200 for the chassis Physcomitrella patens. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 459
    Illegal BsaI.rc site found at 1377


[edit]
Categories
//chassis/eukaryote/ppatens
Parameters
None