Composite

Part:BBa_K1116001

Designed by: Shuguang Peng   Group: iGEM13_Tsinghua-A   (2013-09-10)
Revision as of 05:16, 17 September 2013 by ATsinghua (Talk | contribs)

LacI with miR-FF5 and miR-FF3 target

It was designed as a intermediate product to construct BBa_K1116003 in our project. .We use this part to construct K1116003.However,K1116003 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116003 .The results can be seen in K1116002's experience. 1 So the plasmid K1116001 works because K1116003 have been tested.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1120
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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