Composite

Part:BBa_K1116000

Designed by: Lei Wei   Group: iGEM13_Tsinghua-A   (2013-09-08)
Revision as of 05:14, 17 September 2013 by ATsinghua (Talk | contribs)

LacI with miR-21 and miR-FF3 target

This part is a LacI coding sequence with 4 miR-21 and 4 miR-FF3 target sites so it can be regulated with micro-RNA 21 and FF3.We use this part to construct K1116002.However,K1116002 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116002 .The results can be seen in K1116002's experience. [[1]] So the plasmid K1116000 works because K1116002 have been tested.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1120
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None