Measurement

Part:BBa_K1061014

Designed by: mengyi sun   Group: iGEM13_SYSU-China   (2013-09-16)
Revision as of 04:38, 17 September 2013 by Registry (Talk | contribs)

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eGFP->miRNA-122 target

This is a measurement part. We ligated the 4-copy target of miRNA-122 after eGFP protein. We also get the data of the response of the target toward the dosage of miRNA-122 expression, confirm by fluorence, qRT-PCR and western blot. Using this device, iGEM teams can use it to measure and get enough data for modeling of circuit involve miRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 721
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aeGFP->miRNA-122 target