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Part:BBa_K1116002:Experience

Designed by: Shuguang Peng   Group: iGEM13_Tsinghua-A   (2013-09-10)
Revision as of 01:03, 17 September 2013 by ATsinghua (Talk | contribs) (→‎Applications of BBa_K1116002)


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Applications of BBa_K1116002

In our project, BBa_K1116002 serves as an auxiliary node. Our design as follows (Circuit A). Besides, BBa_K1116003 is used in our control design (Circuit B). The difference between BBa_K1116002 and BBa_K1116003 is the T21 targets.

Figure A.jpg Figure B.jpg

By testing the mean value of EYFP (Enhance Yellow Fluorescent Protein) and the relationship between the EYFP and the DNA copy number, we can know whether the plasmids work or not. In our design, we use mkate (another fluorescent protein), which radiats red lights, as reference gene to reflect copy number. The results as follows. We can distinguish the mean valve of EYFP of circuit A and circuit B from the following figure.In the picture,1, 2,3 are the results of Circuit A and 4,5,6 represent Circuit B.

Figure C.jpg

We can know the miRNA-21 targets at T21,leading the decrease of gene lacI. So the expression of EYFP enhanced, thus the plasmid K1116002 works. Equally, we can prove the K1116003 is right.

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UNIQa6372861def8a87b-partinfo-00000000-QINU UNIQa6372861def8a87b-partinfo-00000001-QINU