DNA

Part:BBa_K1195003:Design

Designed by: Clarice Harrison   Group: iGEM13_BYU_Provo   (2013-08-28)
Revision as of 23:19, 16 September 2013 by Clarlharr (Talk | contribs) (→‎Design Notes)


Promoter Qrr4 + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter Qrr4 from Cholera that is sensitive to the cascade of proteins that are activated when cholera auto-inducers are present was attached onto RFP using soeing PCR. A hard terminator was added to the end of RFP.

Source

Qrr4 is from Vibrio Cholerae. RFP is from the plasmid used to send parts to iGEM.

References