Plasmid
pEBS

Part:BBa_K1019008

Designed by: Boris Dyakov   Group: iGEM13_Toronto   (2013-09-16)
Revision as of 22:51, 16 September 2013 by Registry (Talk | contribs)

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pEBS Expression Plasmid

pEBS (E.coli Biofilm System) is an expression plasmid with an inducible lac promoter. It contains the biobrick prefix and suffix, an RBS, and a unique BseRI cut site which linearizes the vector for use with the Clontech In-Fusion restriction free cloning system. This system allows any gene with the appropriate homology region added to its start and end to be easily swapped into the vector for testing.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1529
    Illegal suffix found in sequence at 1570
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1529
    Illegal SpeI site found at 1571
    Illegal PstI site found at 1585
    Illegal NotI site found at 1535
    Illegal NotI site found at 1578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1529
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1529
    Illegal suffix found in sequence at 1571
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1529
    Illegal XbaI site found at 1544
    Illegal SpeI site found at 1571
    Illegal PstI site found at 1585
    Illegal NgoMIV site found at 3551
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1913
    Illegal BsaI site found at 2981
    Illegal SapI.rc site found at 4558


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