Regulatory

Part:BBa_K1155001:Design

Designed by: Eric Escriva, Sylvie Lautru   Group: iGEM13_Paris_Saclay   (2013-09-16)
Revision as of 16:13, 16 September 2013 by SylvieL (Talk | contribs)

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Promoter region of bphR1 in Pseudomonas pseudoalcaligenes KF707


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


Design Notes

300 bp before the start codon of bphR1


Source

genomic sequence of Pseudomonas pseudoalcaligenes KF707

References

Fujihara, H., Yoshida, H., Matsunaga,T. et al. (2006). Cross-Regulation of Biphenyl- and Salicylate-Catabolic Genes by Two Regulatory Systems in Pseudomonas pseudoalcaligenes KF707. Journal of Bacteriology, 188, 4690-4697.