Coding

Part:BBa_K1159316

Designed by: TU Munich 2013   Group: iGEM13_TU-Munich   (2013-09-16)
Revision as of 12:54, 16 September 2013 by Registry (Talk | contribs)

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SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]

This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing Strep-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Note: A signal peptide is needed for membrane integration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8
    Illegal BsaI.rc site found at 926


[edit]
Categories
//chassis/eukaryote/ppatens
Parameters
None