Regulatory

Part:BBa_K1164004

Designed by: Wilson Lam, Dylan Siriwardena, Yara Abou-Hamde   Group: iGEM13_uOttawa   (2013-09-16)
Revision as of 06:52, 16 September 2013 by Registry (Talk | contribs)

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pTRELX: Modified Gal1 promoter; gal4O replaced w/ tetO; repressable via lacO

This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 465
    Illegal BamHI site found at 553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None