Regulatory
Part:BBa_K1164004
Designed by: Wilson Lam, Dylan Siriwardena, Yara Abou-Hamde Group: iGEM13_uOttawa (2013-09-16)
pTRELX: Modified Gal1 promoter; gal4O replaced w/ tetO; repressable via lacO
This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 465
Illegal BamHI site found at 553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 81
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |