Coding

Part:BBa_K1164002

Designed by: Wilson Lam, Dylan Siriwardena   Group: iGEM13_uOttawa   (2013-09-16)
Revision as of 06:39, 16 September 2013 by Registry (Talk | contribs)

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LacI repressor tagged with yBFP

This is the LacI repressor from E.coli tagged with yeast codon optimized BFP. LacI will bind with high specificity to the the operator lacO and is inhibited in the presence of IPTG. BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm. It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

This is an updated version of BBa_K642001 that is functional in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1044
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None