Composite

Part:BBa_K1157020

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)
Revision as of 01:18, 16 September 2013 by Registry (Talk | contribs)

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Pc-RhlR-pRhl-RhlR-GFPmut3

This biosensor is built to sense C-4 AHL molecules. The constitutive promoter continues to express Rhl receptor(RhlR), and as the Rhl receptor binds with C-4 AHL, it binds with promoter sequence of Rhl and produces more Rhl receptor and reporter GFP which can be detected. The adding of a Rhl receptor before GFP is to induce a positive circuit in order to magnify the signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 1735
    Illegal BsaI.rc site found at 2422


[edit]
Categories
Parameters
None