Composite
Las sensor

Part:BBa_K1157016

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)
Revision as of 17:28, 15 September 2013 by Registry (Talk | contribs)

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Pc-LasR-pLas-BFP

In this biosensor, we use a constitutive promoter to express quorum sensing receptor LasR. When 12-C AHL molecule binds with LasR, it is suppose to regulate the downstream reporter BFP, which is able to detect.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 990
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None