Coding

Part:BBa_K1159100

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-14)
Revision as of 15:30, 15 September 2013 by Earthnut (Talk | contribs)

Autolysis resistant TEV Protease S219V mutant in RFC[25]

TEV Protease from Tabacco Etch Virus (TEV) is a highly specific protease that cleaves the sequence "Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) (ENLYFQ(G/S))" very specifically before the last Gly/Ser. The wildtype version of the protease has a short half-life due to self-inactivation through autolysis. This mutant S219V is autolysis resistant and its enzymatic activity is not affected by the mutation. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316
    Illegal SapI.rc site found at 664


[edit]
Categories
Parameters
None