Coding

Part:BBa_K1159005

Designed by: Louise FUNKE   Group: iGEM13_TU-Munich   (2013-09-14)
Revision as of 15:01, 14 September 2013 by Registry (Talk | contribs)

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Secretory Erythromycin Esterase Type II (IgKappa-SigP_EreB) in RFC[25] N-Part

Erythromycin esterase type II (EreB) is fused with a N-terminal signal peptide for secretion (IgKappa signal peptide from Mus musculus) with also works in moss (Physcomitrella patens). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 262
    Illegal XhoI site found at 672
    Illegal XhoI site found at 1262
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 389


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Categories
Parameters
None