RNA
GFPi

Part:BBa_K1062001

Designed by: David Dinh   Group: iGEM13_UCSF   (2013-09-13)
Revision as of 18:01, 13 September 2013 by Dadinh (Talk | contribs)

Guide RNA (gRNA) target for RFP

This part is a guide RNA (gRNA) that targets GFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project.

Synthetic Circuit.jpg

Conjugation Project

Synthetic Circuit

[edit]
Categories
Parameters
None