Reporter

Part:BBa_K1041000:Experience

Designed by: NRP UEA   Group: iGEM13_NRP-UEA-Norwich   (2013-08-08)
Revision as of 16:20, 13 September 2013 by Holusac (Talk | contribs) (→‎Team NRP-UEA_Norwich 2013)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of BBa_J04450 using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part Bba_K1041002.

The characterisation of the biobrick involved evaluating its effectiveness as a part compated to BBa_J04450.

User Reviews

UNIQa4b3f6dfd543fdff-partinfo-00000000-QINU UNIQa4b3f6dfd543fdff-partinfo-00000001-QINU