Measurement

Part:BBa_K1100011:Design

Designed by: YingQi Lou   Group: iGEM13_Fudan   (2013-09-13)
Revision as of 10:18, 13 September 2013 by Registry (Talk | contribs)

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B0015 terminator efficiency estimation device (with Csy4 system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164


Design Notes

In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence.


Source

The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.The sfGFP is from Ding Yu's Lab at Fudan University.

References