Regulatory

Part:BBa_K1061011

Designed by: mengyi sun   Group: iGEM13_SYSU-China   (2013-09-11)
Revision as of 03:03, 12 September 2013 by Registry (Talk | contribs)

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EF-1alpha promoter

Ef-1alpha promoter is a strong constitutive promoter that drive the expression of elongation factor -1α.It has been widely used in constructing mammalian cell-based circuit, especially useful for construction of long-term circuit in some cells including iPS cells and Jurkat cells in which the strong promoter CMV may be silenced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 440
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aEF-1alpha promoter